CCK-8 Assay:Article Title: Therapeutic Exosomes Carrying VEGFA siRNA Inhibit Pathological Corneal Angiogenesis via PI3K-Akt-Caspase-3 Signaling.
Article Snippet: .. Abbreviations The following abbreviations are used in this manuscript: AGO2 Argonaute-2 Akt Protein kinase B AMT Advanced Microscopy Techniques ANOVA Analysis of variance ATCC American Type Culture Collection BCA Bicinchoninic acid BSA Bovine serum albumin CCK-8 Cell Counting Kit-8 cDNA Complementary DNA CD31/CD63/CD81 Cluster of Differentiation 31/63/81 CCD Charge-coupled device https://doi.org/10.3390/biomedicines14010246 CO2 Carbon dioxide CNV Corneal neovascularization DAPI 4′,6-diamidino-2-phenylindole DMEM Dulbecco’s Modified Eagle Medium DNA Deoxyribonucleic acid ECL Enhanced chemiluminescence EDTA Ethylenediaminetetraacetic acid Exo-Fect Exosome transfection reagent FACS Fluorescence-Activated Cell Sorting FAM Fluorescein amidite FBS Fetal bovine serum FITC Fluorescein isothiocyanate H&E Hematoxylin and eosin HUVECs Human umbilical vein endothelial cells IACUC Institutional Animal Care and Use Committee IgG Immunoglobulin G kDa Kilodalton LN2 Liquid nitrogen LSGS Low-serum growth supplement LSM Laser scanning microscope mRNA Messenger RNA MSC/MSCs Mesenchymal stem cell (s) Nrf2 Nuclear factor erythroid 2–related factor 2 NTA Nanoparticle tracking analysis PBS Phosphate-buffered saline PE Phycoerythrin PI Propidium iodide PI3K Phosphoinositide 3-kinase PVDF Polyvinylidene difluoride qPCR Quantitative polymerase chain reaction RIPA Radioimmunoprecipitation assay RISC RNA-induced silencing complex RNA Ribonucleic acid Rpm Revolutions per minute RT-PCR Reverse transcription polymerase chain reaction SDS Sodium dodecyl sulfate SEM Standard error of the mean SE Standard error siRNA Small interfering RNA TEM Transmission electron microscopy TBST Tris-buffered saline with Tween-20 VEGF Vascular endothelial growth factor VEGFA Vascular endothelial growth factor A VEGFR2 Vascular endothelial growth factor receptor 2 xMAP Multi-Analyte Profiling (Luminex technology) ..
Modification:Article Title: Therapeutic Exosomes Carrying VEGFA siRNA Inhibit Pathological Corneal Angiogenesis via PI3K-Akt-Caspase-3 Signaling.
Article Snippet: .. Abbreviations The following abbreviations are used in this manuscript: AGO2 Argonaute-2 Akt Protein kinase B AMT Advanced Microscopy Techniques ANOVA Analysis of variance ATCC American Type Culture Collection BCA Bicinchoninic acid BSA Bovine serum albumin CCK-8 Cell Counting Kit-8 cDNA Complementary DNA CD31/CD63/CD81 Cluster of Differentiation 31/63/81 CCD Charge-coupled device https://doi.org/10.3390/biomedicines14010246 CO2 Carbon dioxide CNV Corneal neovascularization DAPI 4′,6-diamidino-2-phenylindole DMEM Dulbecco’s Modified Eagle Medium DNA Deoxyribonucleic acid ECL Enhanced chemiluminescence EDTA Ethylenediaminetetraacetic acid Exo-Fect Exosome transfection reagent FACS Fluorescence-Activated Cell Sorting FAM Fluorescein amidite FBS Fetal bovine serum FITC Fluorescein isothiocyanate H&E Hematoxylin and eosin HUVECs Human umbilical vein endothelial cells IACUC Institutional Animal Care and Use Committee IgG Immunoglobulin G kDa Kilodalton LN2 Liquid nitrogen LSGS Low-serum growth supplement LSM Laser scanning microscope mRNA Messenger RNA MSC/MSCs Mesenchymal stem cell (s) Nrf2 Nuclear factor erythroid 2–related factor 2 NTA Nanoparticle tracking analysis PBS Phosphate-buffered saline PE Phycoerythrin PI Propidium iodide PI3K Phosphoinositide 3-kinase PVDF Polyvinylidene difluoride qPCR Quantitative polymerase chain reaction RIPA Radioimmunoprecipitation assay RISC RNA-induced silencing complex RNA Ribonucleic acid Rpm Revolutions per minute RT-PCR Reverse transcription polymerase chain reaction SDS Sodium dodecyl sulfate SEM Standard error of the mean SE Standard error siRNA Small interfering RNA TEM Transmission electron microscopy TBST Tris-buffered saline with Tween-20 VEGF Vascular endothelial growth factor VEGFA Vascular endothelial growth factor A VEGFR2 Vascular endothelial growth factor receptor 2 xMAP Multi-Analyte Profiling (Luminex technology) ..
Transfection:Article Title: Therapeutic Exosomes Carrying VEGFA siRNA Inhibit Pathological Corneal Angiogenesis via PI3K-Akt-Caspase-3 Signaling.
Article Snippet: .. Abbreviations The following abbreviations are used in this manuscript: AGO2 Argonaute-2 Akt Protein kinase B AMT Advanced Microscopy Techniques ANOVA Analysis of variance ATCC American Type Culture Collection BCA Bicinchoninic acid BSA Bovine serum albumin CCK-8 Cell Counting Kit-8 cDNA Complementary DNA CD31/CD63/CD81 Cluster of Differentiation 31/63/81 CCD Charge-coupled device https://doi.org/10.3390/biomedicines14010246 CO2 Carbon dioxide CNV Corneal neovascularization DAPI 4′,6-diamidino-2-phenylindole DMEM Dulbecco’s Modified Eagle Medium DNA Deoxyribonucleic acid ECL Enhanced chemiluminescence EDTA Ethylenediaminetetraacetic acid Exo-Fect Exosome transfection reagent FACS Fluorescence-Activated Cell Sorting FAM Fluorescein amidite FBS Fetal bovine serum FITC Fluorescein isothiocyanate H&E Hematoxylin and eosin HUVECs Human umbilical vein endothelial cells IACUC Institutional Animal Care and Use Committee IgG Immunoglobulin G kDa Kilodalton LN2 Liquid nitrogen LSGS Low-serum growth supplement LSM Laser scanning microscope mRNA Messenger RNA MSC/MSCs Mesenchymal stem cell (s) Nrf2 Nuclear factor erythroid 2–related factor 2 NTA Nanoparticle tracking analysis PBS Phosphate-buffered saline PE Phycoerythrin PI Propidium iodide PI3K Phosphoinositide 3-kinase PVDF Polyvinylidene difluoride qPCR Quantitative polymerase chain reaction RIPA Radioimmunoprecipitation assay RISC RNA-induced silencing complex RNA Ribonucleic acid Rpm Revolutions per minute RT-PCR Reverse transcription polymerase chain reaction SDS Sodium dodecyl sulfate SEM Standard error of the mean SE Standard error siRNA Small interfering RNA TEM Transmission electron microscopy TBST Tris-buffered saline with Tween-20 VEGF Vascular endothelial growth factor VEGFA Vascular endothelial growth factor A VEGFR2 Vascular endothelial growth factor receptor 2 xMAP Multi-Analyte Profiling (Luminex technology) ..
FACS:Article Title: Therapeutic Exosomes Carrying VEGFA siRNA Inhibit Pathological Corneal Angiogenesis via PI3K-Akt-Caspase-3 Signaling.
Article Snippet: .. Abbreviations The following abbreviations are used in this manuscript: AGO2 Argonaute-2 Akt Protein kinase B AMT Advanced Microscopy Techniques ANOVA Analysis of variance ATCC American Type Culture Collection BCA Bicinchoninic acid BSA Bovine serum albumin CCK-8 Cell Counting Kit-8 cDNA Complementary DNA CD31/CD63/CD81 Cluster of Differentiation 31/63/81 CCD Charge-coupled device https://doi.org/10.3390/biomedicines14010246 CO2 Carbon dioxide CNV Corneal neovascularization DAPI 4′,6-diamidino-2-phenylindole DMEM Dulbecco’s Modified Eagle Medium DNA Deoxyribonucleic acid ECL Enhanced chemiluminescence EDTA Ethylenediaminetetraacetic acid Exo-Fect Exosome transfection reagent FACS Fluorescence-Activated Cell Sorting FAM Fluorescein amidite FBS Fetal bovine serum FITC Fluorescein isothiocyanate H&E Hematoxylin and eosin HUVECs Human umbilical vein endothelial cells IACUC Institutional Animal Care and Use Committee IgG Immunoglobulin G kDa Kilodalton LN2 Liquid nitrogen LSGS Low-serum growth supplement LSM Laser scanning microscope mRNA Messenger RNA MSC/MSCs Mesenchymal stem cell (s) Nrf2 Nuclear factor erythroid 2–related factor 2 NTA Nanoparticle tracking analysis PBS Phosphate-buffered saline PE Phycoerythrin PI Propidium iodide PI3K Phosphoinositide 3-kinase PVDF Polyvinylidene difluoride qPCR Quantitative polymerase chain reaction RIPA Radioimmunoprecipitation assay RISC RNA-induced silencing complex RNA Ribonucleic acid Rpm Revolutions per minute RT-PCR Reverse transcription polymerase chain reaction SDS Sodium dodecyl sulfate SEM Standard error of the mean SE Standard error siRNA Small interfering RNA TEM Transmission electron microscopy TBST Tris-buffered saline with Tween-20 VEGF Vascular endothelial growth factor VEGFA Vascular endothelial growth factor A VEGFR2 Vascular endothelial growth factor receptor 2 xMAP Multi-Analyte Profiling (Luminex technology) ..
Fluorescence:Article Title: Therapeutic Exosomes Carrying VEGFA siRNA Inhibit Pathological Corneal Angiogenesis via PI3K-Akt-Caspase-3 Signaling.
Article Snippet: .. Abbreviations The following abbreviations are used in this manuscript: AGO2 Argonaute-2 Akt Protein kinase B AMT Advanced Microscopy Techniques ANOVA Analysis of variance ATCC American Type Culture Collection BCA Bicinchoninic acid BSA Bovine serum albumin CCK-8 Cell Counting Kit-8 cDNA Complementary DNA CD31/CD63/CD81 Cluster of Differentiation 31/63/81 CCD Charge-coupled device https://doi.org/10.3390/biomedicines14010246 CO2 Carbon dioxide CNV Corneal neovascularization DAPI 4′,6-diamidino-2-phenylindole DMEM Dulbecco’s Modified Eagle Medium DNA Deoxyribonucleic acid ECL Enhanced chemiluminescence EDTA Ethylenediaminetetraacetic acid Exo-Fect Exosome transfection reagent FACS Fluorescence-Activated Cell Sorting FAM Fluorescein amidite FBS Fetal bovine serum FITC Fluorescein isothiocyanate H&E Hematoxylin and eosin HUVECs Human umbilical vein endothelial cells IACUC Institutional Animal Care and Use Committee IgG Immunoglobulin G kDa Kilodalton LN2 Liquid nitrogen LSGS Low-serum growth supplement LSM Laser scanning microscope mRNA Messenger RNA MSC/MSCs Mesenchymal stem cell (s) Nrf2 Nuclear factor erythroid 2–related factor 2 NTA Nanoparticle tracking analysis PBS Phosphate-buffered saline PE Phycoerythrin PI Propidium iodide PI3K Phosphoinositide 3-kinase PVDF Polyvinylidene difluoride qPCR Quantitative polymerase chain reaction RIPA Radioimmunoprecipitation assay RISC RNA-induced silencing complex RNA Ribonucleic acid Rpm Revolutions per minute RT-PCR Reverse transcription polymerase chain reaction SDS Sodium dodecyl sulfate SEM Standard error of the mean SE Standard error siRNA Small interfering RNA TEM Transmission electron microscopy TBST Tris-buffered saline with Tween-20 VEGF Vascular endothelial growth factor VEGFA Vascular endothelial growth factor A VEGFR2 Vascular endothelial growth factor receptor 2 xMAP Multi-Analyte Profiling (Luminex technology) ..
Laser-Scanning Microscopy:Article Title: Therapeutic Exosomes Carrying VEGFA siRNA Inhibit Pathological Corneal Angiogenesis via PI3K-Akt-Caspase-3 Signaling.
Article Snippet: .. Abbreviations The following abbreviations are used in this manuscript: AGO2 Argonaute-2 Akt Protein kinase B AMT Advanced Microscopy Techniques ANOVA Analysis of variance ATCC American Type Culture Collection BCA Bicinchoninic acid BSA Bovine serum albumin CCK-8 Cell Counting Kit-8 cDNA Complementary DNA CD31/CD63/CD81 Cluster of Differentiation 31/63/81 CCD Charge-coupled device https://doi.org/10.3390/biomedicines14010246 CO2 Carbon dioxide CNV Corneal neovascularization DAPI 4′,6-diamidino-2-phenylindole DMEM Dulbecco’s Modified Eagle Medium DNA Deoxyribonucleic acid ECL Enhanced chemiluminescence EDTA Ethylenediaminetetraacetic acid Exo-Fect Exosome transfection reagent FACS Fluorescence-Activated Cell Sorting FAM Fluorescein amidite FBS Fetal bovine serum FITC Fluorescein isothiocyanate H&E Hematoxylin and eosin HUVECs Human umbilical vein endothelial cells IACUC Institutional Animal Care and Use Committee IgG Immunoglobulin G kDa Kilodalton LN2 Liquid nitrogen LSGS Low-serum growth supplement LSM Laser scanning microscope mRNA Messenger RNA MSC/MSCs Mesenchymal stem cell (s) Nrf2 Nuclear factor erythroid 2–related factor 2 NTA Nanoparticle tracking analysis PBS Phosphate-buffered saline PE Phycoerythrin PI Propidium iodide PI3K Phosphoinositide 3-kinase PVDF Polyvinylidene difluoride qPCR Quantitative polymerase chain reaction RIPA Radioimmunoprecipitation assay RISC RNA-induced silencing complex RNA Ribonucleic acid Rpm Revolutions per minute RT-PCR Reverse transcription polymerase chain reaction SDS Sodium dodecyl sulfate SEM Standard error of the mean SE Standard error siRNA Small interfering RNA TEM Transmission electron microscopy TBST Tris-buffered saline with Tween-20 VEGF Vascular endothelial growth factor VEGFA Vascular endothelial growth factor A VEGFR2 Vascular endothelial growth factor receptor 2 xMAP Multi-Analyte Profiling (Luminex technology) ..
Saline:Article Title: Therapeutic Exosomes Carrying VEGFA siRNA Inhibit Pathological Corneal Angiogenesis via PI3K-Akt-Caspase-3 Signaling.
Article Snippet: .. Abbreviations The following abbreviations are used in this manuscript: AGO2 Argonaute-2 Akt Protein kinase B AMT Advanced Microscopy Techniques ANOVA Analysis of variance ATCC American Type Culture Collection BCA Bicinchoninic acid BSA Bovine serum albumin CCK-8 Cell Counting Kit-8 cDNA Complementary DNA CD31/CD63/CD81 Cluster of Differentiation 31/63/81 CCD Charge-coupled device https://doi.org/10.3390/biomedicines14010246 CO2 Carbon dioxide CNV Corneal neovascularization DAPI 4′,6-diamidino-2-phenylindole DMEM Dulbecco’s Modified Eagle Medium DNA Deoxyribonucleic acid ECL Enhanced chemiluminescence EDTA Ethylenediaminetetraacetic acid Exo-Fect Exosome transfection reagent FACS Fluorescence-Activated Cell Sorting FAM Fluorescein amidite FBS Fetal bovine serum FITC Fluorescein isothiocyanate H&E Hematoxylin and eosin HUVECs Human umbilical vein endothelial cells IACUC Institutional Animal Care and Use Committee IgG Immunoglobulin G kDa Kilodalton LN2 Liquid nitrogen LSGS Low-serum growth supplement LSM Laser scanning microscope mRNA Messenger RNA MSC/MSCs Mesenchymal stem cell (s) Nrf2 Nuclear factor erythroid 2–related factor 2 NTA Nanoparticle tracking analysis PBS Phosphate-buffered saline PE Phycoerythrin PI Propidium iodide PI3K Phosphoinositide 3-kinase PVDF Polyvinylidene difluoride qPCR Quantitative polymerase chain reaction RIPA Radioimmunoprecipitation assay RISC RNA-induced silencing complex RNA Ribonucleic acid Rpm Revolutions per minute RT-PCR Reverse transcription polymerase chain reaction SDS Sodium dodecyl sulfate SEM Standard error of the mean SE Standard error siRNA Small interfering RNA TEM Transmission electron microscopy TBST Tris-buffered saline with Tween-20 VEGF Vascular endothelial growth factor VEGFA Vascular endothelial growth factor A VEGFR2 Vascular endothelial growth factor receptor 2 xMAP Multi-Analyte Profiling (Luminex technology) ..
Real-time Polymerase Chain Reaction:Article Title: Therapeutic Exosomes Carrying VEGFA siRNA Inhibit Pathological Corneal Angiogenesis via PI3K-Akt-Caspase-3 Signaling.
Article Snippet: .. Abbreviations The following abbreviations are used in this manuscript: AGO2 Argonaute-2 Akt Protein kinase B AMT Advanced Microscopy Techniques ANOVA Analysis of variance ATCC American Type Culture Collection BCA Bicinchoninic acid BSA Bovine serum albumin CCK-8 Cell Counting Kit-8 cDNA Complementary DNA CD31/CD63/CD81 Cluster of Differentiation 31/63/81 CCD Charge-coupled device https://doi.org/10.3390/biomedicines14010246 CO2 Carbon dioxide CNV Corneal neovascularization DAPI 4′,6-diamidino-2-phenylindole DMEM Dulbecco’s Modified Eagle Medium DNA Deoxyribonucleic acid ECL Enhanced chemiluminescence EDTA Ethylenediaminetetraacetic acid Exo-Fect Exosome transfection reagent FACS Fluorescence-Activated Cell Sorting FAM Fluorescein amidite FBS Fetal bovine serum FITC Fluorescein isothiocyanate H&E Hematoxylin and eosin HUVECs Human umbilical vein endothelial cells IACUC Institutional Animal Care and Use Committee IgG Immunoglobulin G kDa Kilodalton LN2 Liquid nitrogen LSGS Low-serum growth supplement LSM Laser scanning microscope mRNA Messenger RNA MSC/MSCs Mesenchymal stem cell (s) Nrf2 Nuclear factor erythroid 2–related factor 2 NTA Nanoparticle tracking analysis PBS Phosphate-buffered saline PE Phycoerythrin PI Propidium iodide PI3K Phosphoinositide 3-kinase PVDF Polyvinylidene difluoride qPCR Quantitative polymerase chain reaction RIPA Radioimmunoprecipitation assay RISC RNA-induced silencing complex RNA Ribonucleic acid Rpm Revolutions per minute RT-PCR Reverse transcription polymerase chain reaction SDS Sodium dodecyl sulfate SEM Standard error of the mean SE Standard error siRNA Small interfering RNA TEM Transmission electron microscopy TBST Tris-buffered saline with Tween-20 VEGF Vascular endothelial growth factor VEGFA Vascular endothelial growth factor A VEGFR2 Vascular endothelial growth factor receptor 2 xMAP Multi-Analyte Profiling (Luminex technology) ..
Radio Immunoprecipitation:Article Title: Therapeutic Exosomes Carrying VEGFA siRNA Inhibit Pathological Corneal Angiogenesis via PI3K-Akt-Caspase-3 Signaling.
Article Snippet: .. Abbreviations The following abbreviations are used in this manuscript: AGO2 Argonaute-2 Akt Protein kinase B AMT Advanced Microscopy Techniques ANOVA Analysis of variance ATCC American Type Culture Collection BCA Bicinchoninic acid BSA Bovine serum albumin CCK-8 Cell Counting Kit-8 cDNA Complementary DNA CD31/CD63/CD81 Cluster of Differentiation 31/63/81 CCD Charge-coupled device https://doi.org/10.3390/biomedicines14010246 CO2 Carbon dioxide CNV Corneal neovascularization DAPI 4′,6-diamidino-2-phenylindole DMEM Dulbecco’s Modified Eagle Medium DNA Deoxyribonucleic acid ECL Enhanced chemiluminescence EDTA Ethylenediaminetetraacetic acid Exo-Fect Exosome transfection reagent FACS Fluorescence-Activated Cell Sorting FAM Fluorescein amidite FBS Fetal bovine serum FITC Fluorescein isothiocyanate H&E Hematoxylin and eosin HUVECs Human umbilical vein endothelial cells IACUC Institutional Animal Care and Use Committee IgG Immunoglobulin G kDa Kilodalton LN2 Liquid nitrogen LSGS Low-serum growth supplement LSM Laser scanning microscope mRNA Messenger RNA MSC/MSCs Mesenchymal stem cell (s) Nrf2 Nuclear factor erythroid 2–related factor 2 NTA Nanoparticle tracking analysis PBS Phosphate-buffered saline PE Phycoerythrin PI Propidium iodide PI3K Phosphoinositide 3-kinase PVDF Polyvinylidene difluoride qPCR Quantitative polymerase chain reaction RIPA Radioimmunoprecipitation assay RISC RNA-induced silencing complex RNA Ribonucleic acid Rpm Revolutions per minute RT-PCR Reverse transcription polymerase chain reaction SDS Sodium dodecyl sulfate SEM Standard error of the mean SE Standard error siRNA Small interfering RNA TEM Transmission electron microscopy TBST Tris-buffered saline with Tween-20 VEGF Vascular endothelial growth factor VEGFA Vascular endothelial growth factor A VEGFR2 Vascular endothelial growth factor receptor 2 xMAP Multi-Analyte Profiling (Luminex technology) ..
Reverse Transcription Polymerase Chain Reaction:Article Title: Therapeutic Exosomes Carrying VEGFA siRNA Inhibit Pathological Corneal Angiogenesis via PI3K-Akt-Caspase-3 Signaling.
Article Snippet: .. Abbreviations The following abbreviations are used in this manuscript: AGO2 Argonaute-2 Akt Protein kinase B AMT Advanced Microscopy Techniques ANOVA Analysis of variance ATCC American Type Culture Collection BCA Bicinchoninic acid BSA Bovine serum albumin CCK-8 Cell Counting Kit-8 cDNA Complementary DNA CD31/CD63/CD81 Cluster of Differentiation 31/63/81 CCD Charge-coupled device https://doi.org/10.3390/biomedicines14010246 CO2 Carbon dioxide CNV Corneal neovascularization DAPI 4′,6-diamidino-2-phenylindole DMEM Dulbecco’s Modified Eagle Medium DNA Deoxyribonucleic acid ECL Enhanced chemiluminescence EDTA Ethylenediaminetetraacetic acid Exo-Fect Exosome transfection reagent FACS Fluorescence-Activated Cell Sorting FAM Fluorescein amidite FBS Fetal bovine serum FITC Fluorescein isothiocyanate H&E Hematoxylin and eosin HUVECs Human umbilical vein endothelial cells IACUC Institutional Animal Care and Use Committee IgG Immunoglobulin G kDa Kilodalton LN2 Liquid nitrogen LSGS Low-serum growth supplement LSM Laser scanning microscope mRNA Messenger RNA MSC/MSCs Mesenchymal stem cell (s) Nrf2 Nuclear factor erythroid 2–related factor 2 NTA Nanoparticle tracking analysis PBS Phosphate-buffered saline PE Phycoerythrin PI Propidium iodide PI3K Phosphoinositide 3-kinase PVDF Polyvinylidene difluoride qPCR Quantitative polymerase chain reaction RIPA Radioimmunoprecipitation assay RISC RNA-induced silencing complex RNA Ribonucleic acid Rpm Revolutions per minute RT-PCR Reverse transcription polymerase chain reaction SDS Sodium dodecyl sulfate SEM Standard error of the mean SE Standard error siRNA Small interfering RNA TEM Transmission electron microscopy TBST Tris-buffered saline with Tween-20 VEGF Vascular endothelial growth factor VEGFA Vascular endothelial growth factor A VEGFR2 Vascular endothelial growth factor receptor 2 xMAP Multi-Analyte Profiling (Luminex technology) ..
Reverse Transcription:Article Title: Therapeutic Exosomes Carrying VEGFA siRNA Inhibit Pathological Corneal Angiogenesis via PI3K-Akt-Caspase-3 Signaling.
Article Snippet: .. Abbreviations The following abbreviations are used in this manuscript: AGO2 Argonaute-2 Akt Protein kinase B AMT Advanced Microscopy Techniques ANOVA Analysis of variance ATCC American Type Culture Collection BCA Bicinchoninic acid BSA Bovine serum albumin CCK-8 Cell Counting Kit-8 cDNA Complementary DNA CD31/CD63/CD81 Cluster of Differentiation 31/63/81 CCD Charge-coupled device https://doi.org/10.3390/biomedicines14010246 CO2 Carbon dioxide CNV Corneal neovascularization DAPI 4′,6-diamidino-2-phenylindole DMEM Dulbecco’s Modified Eagle Medium DNA Deoxyribonucleic acid ECL Enhanced chemiluminescence EDTA Ethylenediaminetetraacetic acid Exo-Fect Exosome transfection reagent FACS Fluorescence-Activated Cell Sorting FAM Fluorescein amidite FBS Fetal bovine serum FITC Fluorescein isothiocyanate H&E Hematoxylin and eosin HUVECs Human umbilical vein endothelial cells IACUC Institutional Animal Care and Use Committee IgG Immunoglobulin G kDa Kilodalton LN2 Liquid nitrogen LSGS Low-serum growth supplement LSM Laser scanning microscope mRNA Messenger RNA MSC/MSCs Mesenchymal stem cell (s) Nrf2 Nuclear factor erythroid 2–related factor 2 NTA Nanoparticle tracking analysis PBS Phosphate-buffered saline PE Phycoerythrin PI Propidium iodide PI3K Phosphoinositide 3-kinase PVDF Polyvinylidene difluoride qPCR Quantitative polymerase chain reaction RIPA Radioimmunoprecipitation assay RISC RNA-induced silencing complex RNA Ribonucleic acid Rpm Revolutions per minute RT-PCR Reverse transcription polymerase chain reaction SDS Sodium dodecyl sulfate SEM Standard error of the mean SE Standard error siRNA Small interfering RNA TEM Transmission electron microscopy TBST Tris-buffered saline with Tween-20 VEGF Vascular endothelial growth factor VEGFA Vascular endothelial growth factor A VEGFR2 Vascular endothelial growth factor receptor 2 xMAP Multi-Analyte Profiling (Luminex technology) ..
Polymerase Chain Reaction:Article Title: Therapeutic Exosomes Carrying VEGFA siRNA Inhibit Pathological Corneal Angiogenesis via PI3K-Akt-Caspase-3 Signaling.
Article Snippet: .. Abbreviations The following abbreviations are used in this manuscript: AGO2 Argonaute-2 Akt Protein kinase B AMT Advanced Microscopy Techniques ANOVA Analysis of variance ATCC American Type Culture Collection BCA Bicinchoninic acid BSA Bovine serum albumin CCK-8 Cell Counting Kit-8 cDNA Complementary DNA CD31/CD63/CD81 Cluster of Differentiation 31/63/81 CCD Charge-coupled device https://doi.org/10.3390/biomedicines14010246 CO2 Carbon dioxide CNV Corneal neovascularization DAPI 4′,6-diamidino-2-phenylindole DMEM Dulbecco’s Modified Eagle Medium DNA Deoxyribonucleic acid ECL Enhanced chemiluminescence EDTA Ethylenediaminetetraacetic acid Exo-Fect Exosome transfection reagent FACS Fluorescence-Activated Cell Sorting FAM Fluorescein amidite FBS Fetal bovine serum FITC Fluorescein isothiocyanate H&E Hematoxylin and eosin HUVECs Human umbilical vein endothelial cells IACUC Institutional Animal Care and Use Committee IgG Immunoglobulin G kDa Kilodalton LN2 Liquid nitrogen LSGS Low-serum growth supplement LSM Laser scanning microscope mRNA Messenger RNA MSC/MSCs Mesenchymal stem cell (s) Nrf2 Nuclear factor erythroid 2–related factor 2 NTA Nanoparticle tracking analysis PBS Phosphate-buffered saline PE Phycoerythrin PI Propidium iodide PI3K Phosphoinositide 3-kinase PVDF Polyvinylidene difluoride qPCR Quantitative polymerase chain reaction RIPA Radioimmunoprecipitation assay RISC RNA-induced silencing complex RNA Ribonucleic acid Rpm Revolutions per minute RT-PCR Reverse transcription polymerase chain reaction SDS Sodium dodecyl sulfate SEM Standard error of the mean SE Standard error siRNA Small interfering RNA TEM Transmission electron microscopy TBST Tris-buffered saline with Tween-20 VEGF Vascular endothelial growth factor VEGFA Vascular endothelial growth factor A VEGFR2 Vascular endothelial growth factor receptor 2 xMAP Multi-Analyte Profiling (Luminex technology) ..
Small Interfering RNA:Article Title: Therapeutic Exosomes Carrying VEGFA siRNA Inhibit Pathological Corneal Angiogenesis via PI3K-Akt-Caspase-3 Signaling.
Article Snippet: .. Abbreviations The following abbreviations are used in this manuscript: AGO2 Argonaute-2 Akt Protein kinase B AMT Advanced Microscopy Techniques ANOVA Analysis of variance ATCC American Type Culture Collection BCA Bicinchoninic acid BSA Bovine serum albumin CCK-8 Cell Counting Kit-8 cDNA Complementary DNA CD31/CD63/CD81 Cluster of Differentiation 31/63/81 CCD Charge-coupled device https://doi.org/10.3390/biomedicines14010246 CO2 Carbon dioxide CNV Corneal neovascularization DAPI 4′,6-diamidino-2-phenylindole DMEM Dulbecco’s Modified Eagle Medium DNA Deoxyribonucleic acid ECL Enhanced chemiluminescence EDTA Ethylenediaminetetraacetic acid Exo-Fect Exosome transfection reagent FACS Fluorescence-Activated Cell Sorting FAM Fluorescein amidite FBS Fetal bovine serum FITC Fluorescein isothiocyanate H&E Hematoxylin and eosin HUVECs Human umbilical vein endothelial cells IACUC Institutional Animal Care and Use Committee IgG Immunoglobulin G kDa Kilodalton LN2 Liquid nitrogen LSGS Low-serum growth supplement LSM Laser scanning microscope mRNA Messenger RNA MSC/MSCs Mesenchymal stem cell (s) Nrf2 Nuclear factor erythroid 2–related factor 2 NTA Nanoparticle tracking analysis PBS Phosphate-buffered saline PE Phycoerythrin PI Propidium iodide PI3K Phosphoinositide 3-kinase PVDF Polyvinylidene difluoride qPCR Quantitative polymerase chain reaction RIPA Radioimmunoprecipitation assay RISC RNA-induced silencing complex RNA Ribonucleic acid Rpm Revolutions per minute RT-PCR Reverse transcription polymerase chain reaction SDS Sodium dodecyl sulfate SEM Standard error of the mean SE Standard error siRNA Small interfering RNA TEM Transmission electron microscopy TBST Tris-buffered saline with Tween-20 VEGF Vascular endothelial growth factor VEGFA Vascular endothelial growth factor A VEGFR2 Vascular endothelial growth factor receptor 2 xMAP Multi-Analyte Profiling (Luminex technology) ..
Transmission Electron Microscopy:Article Title: Therapeutic Exosomes Carrying VEGFA siRNA Inhibit Pathological Corneal Angiogenesis via PI3K-Akt-Caspase-3 Signaling.
Article Snippet: .. Abbreviations The following abbreviations are used in this manuscript: AGO2 Argonaute-2 Akt Protein kinase B AMT Advanced Microscopy Techniques ANOVA Analysis of variance ATCC American Type Culture Collection BCA Bicinchoninic acid BSA Bovine serum albumin CCK-8 Cell Counting Kit-8 cDNA Complementary DNA CD31/CD63/CD81 Cluster of Differentiation 31/63/81 CCD Charge-coupled device https://doi.org/10.3390/biomedicines14010246 CO2 Carbon dioxide CNV Corneal neovascularization DAPI 4′,6-diamidino-2-phenylindole DMEM Dulbecco’s Modified Eagle Medium DNA Deoxyribonucleic acid ECL Enhanced chemiluminescence EDTA Ethylenediaminetetraacetic acid Exo-Fect Exosome transfection reagent FACS Fluorescence-Activated Cell Sorting FAM Fluorescein amidite FBS Fetal bovine serum FITC Fluorescein isothiocyanate H&E Hematoxylin and eosin HUVECs Human umbilical vein endothelial cells IACUC Institutional Animal Care and Use Committee IgG Immunoglobulin G kDa Kilodalton LN2 Liquid nitrogen LSGS Low-serum growth supplement LSM Laser scanning microscope mRNA Messenger RNA MSC/MSCs Mesenchymal stem cell (s) Nrf2 Nuclear factor erythroid 2–related factor 2 NTA Nanoparticle tracking analysis PBS Phosphate-buffered saline PE Phycoerythrin PI Propidium iodide PI3K Phosphoinositide 3-kinase PVDF Polyvinylidene difluoride qPCR Quantitative polymerase chain reaction RIPA Radioimmunoprecipitation assay RISC RNA-induced silencing complex RNA Ribonucleic acid Rpm Revolutions per minute RT-PCR Reverse transcription polymerase chain reaction SDS Sodium dodecyl sulfate SEM Standard error of the mean SE Standard error siRNA Small interfering RNA TEM Transmission electron microscopy TBST Tris-buffered saline with Tween-20 VEGF Vascular endothelial growth factor VEGFA Vascular endothelial growth factor A VEGFR2 Vascular endothelial growth factor receptor 2 xMAP Multi-Analyte Profiling (Luminex technology) ..
Transmission Assay:Article Title: Therapeutic Exosomes Carrying VEGFA siRNA Inhibit Pathological Corneal Angiogenesis via PI3K-Akt-Caspase-3 Signaling.
Article Snippet: .. Abbreviations The following abbreviations are used in this manuscript: AGO2 Argonaute-2 Akt Protein kinase B AMT Advanced Microscopy Techniques ANOVA Analysis of variance ATCC American Type Culture Collection BCA Bicinchoninic acid BSA Bovine serum albumin CCK-8 Cell Counting Kit-8 cDNA Complementary DNA CD31/CD63/CD81 Cluster of Differentiation 31/63/81 CCD Charge-coupled device https://doi.org/10.3390/biomedicines14010246 CO2 Carbon dioxide CNV Corneal neovascularization DAPI 4′,6-diamidino-2-phenylindole DMEM Dulbecco’s Modified Eagle Medium DNA Deoxyribonucleic acid ECL Enhanced chemiluminescence EDTA Ethylenediaminetetraacetic acid Exo-Fect Exosome transfection reagent FACS Fluorescence-Activated Cell Sorting FAM Fluorescein amidite FBS Fetal bovine serum FITC Fluorescein isothiocyanate H&E Hematoxylin and eosin HUVECs Human umbilical vein endothelial cells IACUC Institutional Animal Care and Use Committee IgG Immunoglobulin G kDa Kilodalton LN2 Liquid nitrogen LSGS Low-serum growth supplement LSM Laser scanning microscope mRNA Messenger RNA MSC/MSCs Mesenchymal stem cell (s) Nrf2 Nuclear factor erythroid 2–related factor 2 NTA Nanoparticle tracking analysis PBS Phosphate-buffered saline PE Phycoerythrin PI Propidium iodide PI3K Phosphoinositide 3-kinase PVDF Polyvinylidene difluoride qPCR Quantitative polymerase chain reaction RIPA Radioimmunoprecipitation assay RISC RNA-induced silencing complex RNA Ribonucleic acid Rpm Revolutions per minute RT-PCR Reverse transcription polymerase chain reaction SDS Sodium dodecyl sulfate SEM Standard error of the mean SE Standard error siRNA Small interfering RNA TEM Transmission electron microscopy TBST Tris-buffered saline with Tween-20 VEGF Vascular endothelial growth factor VEGFA Vascular endothelial growth factor A VEGFR2 Vascular endothelial growth factor receptor 2 xMAP Multi-Analyte Profiling (Luminex technology) ..
Electron Microscopy:Article Title: Therapeutic Exosomes Carrying VEGFA siRNA Inhibit Pathological Corneal Angiogenesis via PI3K-Akt-Caspase-3 Signaling.
Article Snippet: .. Abbreviations The following abbreviations are used in this manuscript: AGO2 Argonaute-2 Akt Protein kinase B AMT Advanced Microscopy Techniques ANOVA Analysis of variance ATCC American Type Culture Collection BCA Bicinchoninic acid BSA Bovine serum albumin CCK-8 Cell Counting Kit-8 cDNA Complementary DNA CD31/CD63/CD81 Cluster of Differentiation 31/63/81 CCD Charge-coupled device https://doi.org/10.3390/biomedicines14010246 CO2 Carbon dioxide CNV Corneal neovascularization DAPI 4′,6-diamidino-2-phenylindole DMEM Dulbecco’s Modified Eagle Medium DNA Deoxyribonucleic acid ECL Enhanced chemiluminescence EDTA Ethylenediaminetetraacetic acid Exo-Fect Exosome transfection reagent FACS Fluorescence-Activated Cell Sorting FAM Fluorescein amidite FBS Fetal bovine serum FITC Fluorescein isothiocyanate H&E Hematoxylin and eosin HUVECs Human umbilical vein endothelial cells IACUC Institutional Animal Care and Use Committee IgG Immunoglobulin G kDa Kilodalton LN2 Liquid nitrogen LSGS Low-serum growth supplement LSM Laser scanning microscope mRNA Messenger RNA MSC/MSCs Mesenchymal stem cell (s) Nrf2 Nuclear factor erythroid 2–related factor 2 NTA Nanoparticle tracking analysis PBS Phosphate-buffered saline PE Phycoerythrin PI Propidium iodide PI3K Phosphoinositide 3-kinase PVDF Polyvinylidene difluoride qPCR Quantitative polymerase chain reaction RIPA Radioimmunoprecipitation assay RISC RNA-induced silencing complex RNA Ribonucleic acid Rpm Revolutions per minute RT-PCR Reverse transcription polymerase chain reaction SDS Sodium dodecyl sulfate SEM Standard error of the mean SE Standard error siRNA Small interfering RNA TEM Transmission electron microscopy TBST Tris-buffered saline with Tween-20 VEGF Vascular endothelial growth factor VEGFA Vascular endothelial growth factor A VEGFR2 Vascular endothelial growth factor receptor 2 xMAP Multi-Analyte Profiling (Luminex technology) ..
|
